Hadi Tavakkoli Kazeroni; Seyyed Ebrahim Hosseini; Mehrdad Shariati
Volume 21, Issue 3 , July and August 2014, , Pages 514-521
Abstract
Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research ...
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Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research has been done on the hops. Therefore, this study aimed to evaluate the effect of hops extract on sexual hormone levels and sexual dynastic cells in Syrian adult male mice.
Materials and Methods: This experimental study was performed on 40 Syrian adult male mice that were divided into 5 groups of 8: two controls groups and three experimental groups receiving various doses of hops extract (50, 100 and 150 mg/kg). Administrations were done by gavage for 35 days. At the end of the treatment period, blood samples were taken from the heart of animals and testosterone, estrogen and progesterone levels was measured. Also, after isolation of mouse testis, the number of spermatogonia, spermatocytes and spermatid were counted. Data were analyzed using ANOVA and Tukey statistical tests.
Results: The results showed that hop caused a significant increase in estrogen and testosterone levels and spermatogonia and spermatocytes cells number; but has no effect on progesterone levels.
Conclusion: Hops extract, possibly by having phytoestrogen compounds and by stimulating LH secretion, increases estrogen and testosterone levels, and spermatogonia, spermatocytes and spermatid cells number. Therefore, further investigation on hops can utilize to help infertile men.
Dorna Ajdari; Mehrdad Shariati; Mokhtar Mokhtari
Volume 20, Issue 2 , May and June 2013, , Pages 133-141
Abstract
Introduction and Aim: Nowedays vitamin A consumption to compensate for the lack of it in the body or healing of skin disorders without considering respective side effects is so common.In this research the protective effect of saffron hydro alcoholic extracts on liver enzymes following vitamin A ...
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Introduction and Aim: Nowedays vitamin A consumption to compensate for the lack of it in the body or healing of skin disorders without considering respective side effects is so common.In this research the protective effect of saffron hydro alcoholic extracts on liver enzymes following vitamin A toxicity was investigated .
Materials and Methods: In the experimental study 48 rats were divided into 6 groups of 8 , namely control, sham and 4experimental.Control group didn’t received anythings , sham group received only distilled water. The experimental group 1 Number 1 received saffron hydro alcoholic extract with 50mg/kg dose for 15 days.Experimental groups 3, 4, 5, initially received vitamin A 50000IU for 15 days. The experimental groups 4,5 received saffron hydro alcoholic extract with 50,80 (mg/kg )dose for 15 days. At the end of this period ,blood was collected from the hearts for measuring the amounts of AST ,ALT and ALP.Obtained data were statistically analyzed using statistical tests including one way variance analysis and tukey test . P
Mehrdad Shariati; Arash Esfandiari; Mehrdad Modarresi; Zahra Rahmani
Volume 19, Issue 1 , March and April 2012, , Pages 34-41
Abstract
Background: Extensive supply of herbal products in today’s societies in normal diet or as medications necessitates conduction of toxicological, histological, and hormonal studies. Awareness of the effects of medicinal plants and their inclusion in the diet can contribute to reproductive health. Since ...
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Background: Extensive supply of herbal products in today’s societies in normal diet or as medications necessitates conduction of toxicological, histological, and hormonal studies. Awareness of the effects of medicinal plants and their inclusion in the diet can contribute to reproductive health. Since Mentha pulegium is widely used in pharmaceutical and food industries due to its strong fragrance and beneficial therapeutic effects, this study examined the effects of hydro-alcoholic extract of Mentha pulegium leaves on the function of pituitary-testicular axis.
Materials and Methods: In this experimental study, 40 male rats of Wistar strain weighing about 190 220 g were divided into five groups of eight. The control group received nothing, the sham group received distilled water and ethanol, and the experimental groups received 400, 800, and 1600 mg/kg b.w. hydro-alcoholic extract of Mentha pulegium leaves intraperitoneally. The experiment period was 28 days.
Results: In this study, testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels showed a significant decrease compared to the control group. Furthermore, the number of spermatozoa and Leydig cells had declined in the experimental groups receiving the high dose of the extract. Histological examinations also revealed extensive degenerative changes in the germinal epithelium and spermatogenesis arrest in the experimental groups receiving the high dose of the extract.
Conclusion: Hydro-alcoholic extract of Mentha pulegium leaves in low dose had a significant decreasing effect on plasma total testosterone, FSH, and LH levels. The effects of this extract on histological changes of testis were dose-related.
Mehrdad Shariati; Mokhtar Mokhtari; Somayyeh Negahban
Volume 16, Issue 2 , July and August 2009, , Pages 72-78
Abstract
Background and Purpose: Lamotrigine is a novel potential antiepileptic drug which is not structurally dependent on the current antiepileptic drugs; it has fewer side effects too. The present study intends to assess the effects of Lamotrigine on the antinociceptive activity induced by morphine by using ...
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Background and Purpose: Lamotrigine is a novel potential antiepileptic drug which is not structurally dependent on the current antiepileptic drugs; it has fewer side effects too. The present study intends to assess the effects of Lamotrigine on the antinociceptive activity induced by morphine by using the formalin test as a test of nociception. Methods and Materials: This experimental study was conducted on 56 adult male rats. The animals were divided into seven groups of eight rats considered as one control one sham and five experimental groups (one group receiving 2mg/Rat of morphine alone three groups receiving 2mg/Rat morphine accompanied by Lamotrigine in doses of 25 50 and 75 mg/Rat and the fifth group receiving only 75 mg/Rat of Lamotrigine alone). The drugs were intraperitoneally injected 15 minutes before the formalin test. The sham group received the same volume of physiologic serum and the control group received formalin alone. The study data were analyzed in SPSS using Tukey and Kruskal Wallis test; level of significance was considered to be 0.05. Results: Mean pain score following the injection of maximum dose of lamotrigine together with morphine was 0.08±0.001 which indicated more local analgesic effects at the acute phase in comparison with the injection of morphine alone (0.11±0.0005) (p
Mikhtar Mokhtari; Mehrdad Shariati; Sadaf Rastegar
Volume 16, Issue 1 , March and April 2009, , Pages 12-17
Abstract
Background and Purpose: While aloe vera has the capability of reducing libido regulating menstruation and being widely used there is scarce information on the effect of its alcoholic extract on the functions of pituitary-gonad axis. Therefore the present study aims at investigating the effect of Aloe ...
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Background and Purpose: While aloe vera has the capability of reducing libido regulating menstruation and being widely used there is scarce information on the effect of its alcoholic extract on the functions of pituitary-gonad axis. Therefore the present study aims at investigating the effect of Aloe vera alcoholic extract on spermatogenesis in male rat. Methods and Materials: In this experimental; study 45 male Wistar rats were divided in to five groups including 1 control 1 sham and three experimental groups. Each rat in the experimental groups was treated orally with 50 100 and 150 (mg/kg) doses of Aloe vera alcoholic extract for twenty one days after the extract was prepared via percolation. The sham group received distilled water and alcohol; while control rats received nothing. After twenty one days blood samples were taken from each group directly from ventricles. Blood samples were used to measure the concentration changes of LH FSH and testosterone hormones. Obtained data were statistically analyzed using Tukey test and LSD. Results: Concentrations of FSH in the average and maximum experimental groups (2.963.51 and 2.154.85 respectively) significantly reduced in comparison with the control group (3.112.16). Also concentrations of testosterone in the average and maximum experimental groups (6.183.41 and 3.925.27 respectively) significantly reduced in comparison with the control group (6.857.19). However no significant changes were observed in the concentrations of LH across experimental and control groups. Conclusion: Based on the results Aloe vera extract probably has anti-gonadotropin and antiandrogenic characteristics because of beta-sitosterol ascorbic acid P-coumaric acid coumarin and aloe emodin. It can cause disorders in the secretion of gonadotropin hormone (FSH) and testosterone.
Mehrdad Shariati; Laleh Khodaparast; Mokhtar Mokhtari
Volume 15, Issue 2 , July and August 2008, , Pages 73-81
Abstract
Background and Purpose: Liver is one of the vital organs which has a key role in regulating most physiologic mechanisms and any disorder in hepatic function can cause a series of physiologic and anatomical disorders and still other diseases. Since Mentha pulegium has been used extensively in traditional ...
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Background and Purpose: Liver is one of the vital organs which has a key role in regulating most physiologic mechanisms and any disorder in hepatic function can cause a series of physiologic and anatomical disorders and still other diseases. Since Mentha pulegium has been used extensively in traditional medicine the aim of the present investigation is to study of the effects of the Hudro-Alcoholic Extract of the Mentha Pulegium Leaves on hepatic functions. Methods and Materials: In this experimential study 50 Wistar male rats (about 220- 230 g) were used in five groups of tens including: control group receiving nothing sham group receiving distilled water and 3 experimental groups receiving 400 800 and 1600 (mg/kg) of the Hydro-Alcohol Extract of Mentha Pulegium Leaves respectively. The obtained data were analyzed by t-test using SPSS and Microsoft Excel. Results: According to the results there is a significant reduction in the level of ALT AST in experimental groups. In addition the level of ALP showed a significant increase. Regarding albumin and total protein there has been a decline in the experimental groups receiving the highest dose of the extract and asignifilant reduction in the total body weight in proportion to the increased dosage of the extract. Conclusion: The Hydro-Alcoholic extract of mentha pulegium leaves probably has a significant effect on hepatic function. This effect may be due to the presence of Flavonoids (Hesperidin diosmine) antioxidant tannin and pulegone in the extract. This effect is reflected in the reduction of ALT AST and the increase in ALP. Also it can be attributed to the decrease in the hepatic serum level of the albumin and total protein